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Image Search Results
Journal: PLoS ONE
Article Title: Real-Time Analysis and Visualization for Single-Molecule Based Super-Resolution Microscopy
doi: 10.1371/journal.pone.0062918
Figure Lengend Snippet: ( A ) 3D test patterns made of 40 sunburst alternating black and white stripes, used for single molecule based super-resolution microscopy simulations. The pattern is 1 µm thick, with consecutive stripes distant from 50 nm in the axial direction. ( B ) Diffraction limited image of the test pattern. ( C ) Example of isolated single point emitters located inside the test pattern, convolved with 3D astigmatic point spread function (PSF). ( D–F ) Test pattern reconstruction performed by WaveTracer software for 1000, 200 and 100 photons/molecule respectively. ( G–I ) Test pattern reconstruction performed by RapidSTORM software for 1000, 200 and 100 photons/molecule respectively. ( J–L ) Test pattern reconstruction performed by QuickPALM software for 1000, 200 and 100 photons/molecule respectively. Scale bar is 1 µm.
Article Snippet: Images were analyzed and reconstructed online using the
Techniques: Super-Resolution Microscopy, Isolation, Software