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MetaMorph Inc wavetracer module
( A ) 3D test patterns made of 40 sunburst alternating black and white stripes, used for single molecule based super-resolution microscopy simulations. The pattern is 1 µm thick, with consecutive stripes distant from 50 nm in the axial direction. ( B ) Diffraction limited image of the test pattern. ( C ) Example of isolated single point emitters located inside the test pattern, convolved with 3D astigmatic point spread function (PSF). ( D–F ) Test pattern reconstruction performed by <t>WaveTracer</t> software for 1000, 200 and 100 photons/molecule respectively. ( G–I ) Test pattern reconstruction performed by RapidSTORM software for 1000, 200 and 100 photons/molecule respectively. ( J–L ) Test pattern reconstruction performed by QuickPALM software for 1000, 200 and 100 photons/molecule respectively. Scale bar is 1 µm.
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Image Search Results


( A ) 3D test patterns made of 40 sunburst alternating black and white stripes, used for single molecule based super-resolution microscopy simulations. The pattern is 1 µm thick, with consecutive stripes distant from 50 nm in the axial direction. ( B ) Diffraction limited image of the test pattern. ( C ) Example of isolated single point emitters located inside the test pattern, convolved with 3D astigmatic point spread function (PSF). ( D–F ) Test pattern reconstruction performed by WaveTracer software for 1000, 200 and 100 photons/molecule respectively. ( G–I ) Test pattern reconstruction performed by RapidSTORM software for 1000, 200 and 100 photons/molecule respectively. ( J–L ) Test pattern reconstruction performed by QuickPALM software for 1000, 200 and 100 photons/molecule respectively. Scale bar is 1 µm.

Journal: PLoS ONE

Article Title: Real-Time Analysis and Visualization for Single-Molecule Based Super-Resolution Microscopy

doi: 10.1371/journal.pone.0062918

Figure Lengend Snippet: ( A ) 3D test patterns made of 40 sunburst alternating black and white stripes, used for single molecule based super-resolution microscopy simulations. The pattern is 1 µm thick, with consecutive stripes distant from 50 nm in the axial direction. ( B ) Diffraction limited image of the test pattern. ( C ) Example of isolated single point emitters located inside the test pattern, convolved with 3D astigmatic point spread function (PSF). ( D–F ) Test pattern reconstruction performed by WaveTracer software for 1000, 200 and 100 photons/molecule respectively. ( G–I ) Test pattern reconstruction performed by RapidSTORM software for 1000, 200 and 100 photons/molecule respectively. ( J–L ) Test pattern reconstruction performed by QuickPALM software for 1000, 200 and 100 photons/molecule respectively. Scale bar is 1 µm.

Article Snippet: Images were analyzed and reconstructed online using the WaveTracer module integrated into Metamorph software, running on a Intel Xeon E5645@2.4 GHz personal computer (Dell) equipped with a Nvidia Quadro 4000 graphic card.

Techniques: Super-Resolution Microscopy, Isolation, Software